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2019-3-15 · Tissues were grinded with liquid nitrogen and the collected cells were washed by PBS. They were lysed using RIPA lysis buffer with 1 mM PMSF, 14 mM DTT, 2 μg/mL Aprotinin, 5 μg/mL Leupeptin and 1 μg/mL Pepstain. The lysates were broke by ultrasonic crusher, then centrifuged at …

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The Open Mechanical Engineering Journal :: Volume 8

Multi-Objective Optimization of Impact Crusher Rotor Based on Response Surface Methodology The Open Mechanical Engineering Journal, 2014, 8: 519-525 Li-Mei Zhao, Lun-Jun Chen, Feng He, Yu Luo Electronic publication date 24/12/2014 [DOI: 10.2174/1874155X01408010519]

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2020-11-1 · Incubation with 1 mM FFA led to a large amount of lipid accumulation in HepG2 cells. Compared with the control group, the absorbance (485 nm) value and intracellular red lipid droplet aggregation, presented as large round particle, were significantly higher in the model group (Figure 2(a,b)) pared with the model group, the absorbance (485 nm) at 25 μM and 50 μM G-Rg1 groups …

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2021-5-4 · cell crusher and centrifuged by ultracentrifuge to obtain protein, protein concentration was measured by BCA method, electrophoresis of protein (30 μg/well) in SDS-PAGE electrophoresis solution (concentration 10% gel), PVDF The membrane (Millpore, USA) was transferred to the membrane and incubated with the corresponding antibody (Table 1)

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Trimetazidine protects against myocardial ischemia ...

2018-6-29 · Trimetazidine (TMZ) has been demonstrated to have protective effects against myocardial ischemia/reperfusion (MI/R) injury. In the present study, we investigated the effects and the underlying mechanisms of TMZ on autophagy during MI/R in vivo and in vitro. In the in vivo study, an animal model of MI/R was induced by coronary occlusion. TMZ (20 mg/kg/day) protected the rat hearts from MI/R ...

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The Open Mechanical Engineering Journal

Join Our Editorial Board News release date: March 29, 2018 Description: The Open Mechanical Engineering Journal is an Open Access online journal, which publishes research articles, reviews, letters, case reports and guest-edited single topic issues in all areas of mechanical engineering.

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In vivo hepatoprotective activity and the underlying ...

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The Open Mechanical Engineering Journal

Multi-Objective Optimization of Impact Crusher Rotor Based on Response Surface Methodology Li-Mei Zhao, Lun-Jun Chen, Feng He and Yu Luo The Open Mechanical Engineering Journal, 2014; 8: 519. Electronic Publication Date: December 24, 2014